Efficient, Low-Cost Nucleofection of Passaged Chondrocytes

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Efficient, Low-Cost Nucleofection of Passaged Chondrocytes

Nucleofection of chondrocytes has been shown to be an adequate method of transfection. Using Amaxa's nucleofection system, transfection efficiencies up to 89% were achievable for vector (pmaxGFP) and 98% for siRNA (siGLO) into passaged chondrocytes. However, such methods rely on costly commercial kits with proprietary reagents limiting its use in basic science labs and in clinical translation. ...

متن کامل

High Efficiency Low Cost Fibroblast Nucleofection for GMP Compatible Cell-based Gene Therapy

Background: Dermal fibroblast is a powerful tool for the study of ex vivo DNA delivery in development of both cell therapy and tissue engineering products. Using genetic modification, fibroblasts can be diversely adapted and made suitable for clinical gene therapy. In this study, we first compared several non-viral transfection methods including nucleofection in rat and human primary dermal fib...

متن کامل

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Macrophages, as key players of the innate immune response, are at the focus of research dealing with tissue homeostasis or various pathologies. Transfection with siRNA and plasmid DNA is an efficient tool for studying their function, but transfection of macrophages is not a trivial matter. Although many different approaches for transfection of eukaryotic cells are available, only few allow reli...

متن کامل

A comparison of primary and passaged chondrocytes for use in engineering the temporomandibular joint.

OBJECTIVE This study examines the tissue engineering potential of passaged (P3) and primary (P0) articular chondrocytes (ACs) and costal chondrocytes (CCs) from skeletally mature goats for use in the temporomandibular joint (TMJ). DESIGN These four cell types were assembled into scaffoldless tissue engineered constructs and cultured for 4 wks. The constructs were then tested for cell, collage...

متن کامل

Efficient Edge Detection on Low-Cost FPGAs

Improving the efficiency of edge detection in embedded applications, such as UAV control, is critical for reducing system cost and power dissipation. Field programmable gate arrays (FPGA) are a good platform for making improvements because of their specialised internal structure. However, current FPGA edge detectors do not exploit this structure well. A new edge detection architecture is propos...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: CARTILAGE

سال: 2015

ISSN: 1947-6035,1947-6043

DOI: 10.1177/1947603515609399